escc cell lines kyse 150  (ATCC)

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ inhibits ESCC viability and colony formation. ( A ) Viability of KYSE-70, KYSE-450, and KYSE-510 cells treated with varying concentrations of IQ (0, 200, 400, and 800 µM) and CDDP (25 µM) for 0, 24, 48, 72, and 96 h. Cell viability was measured using the CCK-8 assay. ( B ) Morphology of KYSE-70, KYSE-450, and KYSE-510 cells treated with CDDP (25 µM) and IQ at 0, 400, and 800 µM for 48 h, observed under light microscopy (Scale bar = 316.5 µM). ( C ) Colony formation of KYSE-70, KYSE-450, and KYSE-510 cells treated with 0, 400, and 800 µM IQ for 14 days. The colonies were stained with crystal violet for quantification. ( D ) Quantified colonies of KYSE-70, KYSE-450, and KYSE-510 cells. The colony counts were normalized to the untreated control. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to the control group.

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: CCK-8 Assay, Light Microscopy, Staining, Control

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ suppresses ESCC migration and invasion by modulating the expression of EMT-related proteins. ( A ) Representative images of transwell migration of KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 24 h. The migrated cells were stained with crystal violet and observed under a light microscope (Scale bar = 316.5 µM). ( B ) Representative images of ESCC cell invasion through the Matrigel-coated membrane following IQ treatment. IQ inhibited cell invasion in a dose-dependent manner (Scale bar = 316.5 µM). ( C ) Quantification of the cell area for each treatment condition’s migration and invasion of KYSE-70, KYSE-450, and KYSE-510 cells. ( D ) Western blot analysis of EMT-related proteins in KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 48 h. Protein expression levels of E-cadherin (epithelial marker), N-cadherin, and vimentin (mesenchymal markers) were assessed. β-actin was used as the loading control. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to the control group.

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: Migration, Expressing, Staining, Light Microscopy, Membrane, Western Blot, Marker, Control

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ induces ROS generation and promotes autophagy in ESCC cells. ( A ) Representative images of ROS in KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 48 h. The cells were stained with DCFH-DA (green fluorescence) and imaged by fluorescence microscopy (scale bar = 25 µM). ( B ) Western blot analysis of the antioxidant proteins catalase, SOD1, and SOD2 in KYSE-70, KYSE-450, and KYSE-510 cells after treatment with 0, 400, and 800 µM IQ. ( C ) Viability of ESCC cells cotreated with IQ and the autophagy inhibitor NAC (500 µM). ( D ) IF staining of LC3 and p62 in KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 48 h. Green fluorescence indicates LC3 puncta, while blue fluorescence represents DAPI-stained nuclei (Scale bar = 161.2 µM). ( E ) Western blot analysis of the autophagy-related proteins LC3-II/I and p62 in KYSE-70, KYSE-450, and KYSE-510 cells after treatment with 0, 400, and 800 µM IQ. ( F ) Viability of ESCC cells cotreated with IQ and the autophagy inhibitor CQ (20 µM). Data are presented as mean ± SD with the following significance levels compared to the control group: ** p < 0.01, *** p < 0.001.

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: Staining, Fluorescence, Microscopy, Western Blot, Control

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ induces apoptosis in ESCC cells by modulating the AKT/mTOR signaling pathway. ( A ) Flow cytometry of KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 48 h. Annexin V-FITC/PI staining was used to detect early and late apoptotic cells. ( B ) Quantification of apoptotic KYSE-70, KYSE-450, and KYSE-510 cells. ( C ) Western blot analysis of apoptosis-related proteins in KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM. ( D ) Western blot analysis of key proteins in the AKT/mTOR signaling pathway, including AKT, p-AKT, mTOR, and p-mTOR, in KYSE-70, KYSE-450, and KYSE-510 cells treated with IQ at 0, 400, and 800 µM for 48 h. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to the control group.

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: Flow Cytometry, Staining, Western Blot, Control

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ suppresses the growth of ESCC tumors and modulates their characteristics in vivo. ( A ) Body weights of mice treated with IQ at doses of 0, 10, and 20 mg/kg over 28 days. ( B ) Representative images of tumor tissues excised from mice after 28 days of treatment with IQ at 0, 10, and 20 mg/kg. ( C ) Tumor growth curve showing average tumor volumes of the groups treated with IQ at 0, 10, and 20 mg/kg over 28 days. ( D ) Tumor weights after treatment with IQ at different doses. ( E ) Western blot analysis of key signaling proteins (p-AKT, AKT, p-mTOR, and mTOR) and the autophagy marker LC3-II in tumor tissues. ( F ) IHC analysis of tumor tissues for signaling proteins and Ki-67 (Scale bar = 80 µM). ( G ) H&E staining of tissues harvested from the control and IQ-treated groups. Scale bar = 100 µM. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: In Vivo, Western Blot, Marker, Staining, Control

Journal: Antioxidants

Article Title: Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

doi: 10.3390/antiox14060694

Figure Lengend Snippet: IQ inhibits esophageal squamous cell carcinoma progression by inducing excessive autophagy through the regulation of the AKT/mTOR pathway. The upward and downward arrows represent increased and decreased protein expression levels, respectively. (Created in BioRender. HUANG, K. (2025) https://BioRender.com/y53f756 accessed on 6 March 2025).

Article Snippet: The ESCC cell lines KYSE-510, KYSE-450, and KYSE-70 were obtained from the China-US (Henan) Hormel Cancer Institute (Zhengzhou, China).

Techniques: Expressing